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Background: Eyes are the window to the soul, eye is so closely linked neurologically to the brain that you might call it the only part of our brain you can see from the outside. It seems to hold vital clues to our brain function. Therefore the study was conducted to know the association between colour of eye relate with personality and mental health Methods: Study examined personality of 300 subjects selected purposively. The study was conducted on 143 were normal subjects (77male & 66 female) GHQ negative (score less than 3) subjects from the community formed the normal group for the study and 157 indoor & outdoor   psychiatric patients (suffering with three type of disorder like schizophrenics, bipolar and conversion) (102 male & 55 female) admitted in Nirwan neuropsychiatric clinic Lucknow & Bareilly mental hospital, Bareilly U.P. India. Age ranging between 20-30, 40-50 & 60 and above served as participants. Sentence completion test (SCT) was administered on all the included subjects. Result: Light brown color of eyes male had high psychiatric morbidity (68.47%) then female. Conclusion: Colour of eye can be indicate the personality trait of human being black colour of eyes indicate negative traits of personality in female.