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Tobacco use is a multi- dimensional and a worldwide problem with an alarming increase in its incidences. Adolescence continuing use of tobacco is major public health problem in India. The present study was attempted to study the personality traits of tobacco users (TU) than tobacco non-users (TNU) students. The purposive sampling method was adopted to select the sample. The sample consisted of 100 subject with tobacco users (n=50) and non-users (n=50) were selected from different schools and colleges of Jamnagar city of Gujarat state. The tools used were Eysenck’s Personality Inventory, gujarati version. Student-T test was used to analyses the data. The present study revealed that there is significant difference between student tobacco users and non-users on Neuroticism and Extraversion dimensions of personality. Student tobacco users score higher on both dimensions of personality than non-users. Conclusion: when tobacco prevention and intervention program are developed, these personality traits should be consider in addition to physiological aspects.