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Work motivation \"is a set of energetic forces that originate both within as well as beyond an individual\'s being, to initiate work-related behavior, and to determine its form, direction, intensity, and duration”. Motivation is the driving force inside a person which triggers them to action. It emerges out of persons\' needs, perceived goals, values, intentions and expectations for a better quality of life. In the present study, an attempt is made to find out the influence of work motivation on Quality of life of railway employees. This was done by taking a sample size 300 of south western railway employees of both male and female staffs. They were administered work motivation questionnaire developed by Agarwal (2012) and quality of life scale by Dubey and Dwivedi (2009).  Two-way ANOVA was employed to find out the influence of work motivation, gender, and age on quality of life of railway employees.  The findings show that as the work motivation level increased, the QOL of employees also increased linearly and significantly. Gender as such did not have significant influence over QOL. Age of the employees did not have a significant influence on work motivation.