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Organization is just like structure as well as human body. It has some essential features e.g. structure, process, relationship, authority, responsibility, performance, individuals group behaviour, which is used to man and woman in different status with specific relations. Present study aimed and objective examine the relationship between Nurses services reference to Gender and experience of Organizational health for reaching the unreachable. The Nurses working of organizationl health in implies not only his bicultural approach but she’s wide approaches of ‘tricultural’ during service like values of stress, tension, anxiety, conflict etc. of different places with concerned peoples. Fordyce and well, (1971), discussed detailed characteristics of healthy and unhealthy organizations. Schein, (1973). \"The researcher has used two Independent variables of present study as well as The variables namely (A) Types of Gender (B) types of experiences as a factors effected of organizational health. The perceived organizational health Inventory Constructed & standardized by Miles M.B (1973), Dr.Makvana (2010) and Samira Khalifa was used in (2017). The scale consisted in total statement was 40, five type key answer as a completely agreed, Agreed to a great extent, some extent, little extent to completely disagree.. A sample of 240 respondent’s viz. Male and female was selected from Navasari district different primary health centre and CHC (Chikhali-168 to 170,Gandevi-171,Navasari-172-73,Vasada-174-75, and Jalalpur-176-77) situated at Navasari district. Hence, this gives rise to a variables of Gender and experience for the analysis of the data. Statistical technique like ‘t’ was resorted in order to get the results.