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This study was conducted to investigate the difference in Life Satisfaction and Frustration in patients of cancer and tuberculosis. Total 120 samples of cancer and tuberculosis patients were taken from Sir. T. Government hospital from Bhavnagar city (Gujarat). Their samples were taken along with the data collected with the help of ‘Life Satisfaction Scale.’ This scale was constructed by Dr. Singh P. and Joseph G. (1996). The original Hindi scale was translated and standardized in Gujarati by H. D. Pancholi (2009). And ‘Frustration Scale.’ This scale was constructed by Dr. N. S. Chauhan and Dr. G. P. Tiwari (1972). The original Hindi scale was translated and standardized in Gujarati by J. A. Vegad (2015). The data was used to obtain the Life Satisfaction and Frustration measurement of the Subjects. The collected data was statistically analyzed with the help of ‘F’ and ‘r’ test. The results show that there is no significant difference between Cancer and Tuberculosis patients on Life Satisfaction (F = 3.08). There is no significant difference between Male and Female patients on Life Satisfaction (F = 2.38). There is significant difference between type of disease and gender on Life Satisfaction (F = 7.54). There is significant difference between Cancer and Tuberculosis patients on Frustration (F = 14.79). There is significant difference between Male and Female patients on Frustration (F = 11.97). There is no significant difference between type of disease and gender on Frustration (F = 2.66).