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Spirituality may refer as a subjective feeling and experiences that occur both within and outside of traditional religious systems that influence various domains of life e.g. physical health, mental health, optimism, resilience and quality of life. The aim of the present study is to explore the relation between spirituality and quality of life in male and female hostlers. To accomplish the goal of study, we selected 100 girls and 100 boys post graduate students from Aligarh Muslim University through random sampling method. The entire participants were hostlers whom age ranges from 18-24 years. We applied Daily spiritual experience scale (DSES) and WHOQOL-BREF on the participants to collect data. We analyzed data with the help of t-test and Pearson product moment co-efficient of correlation. The results indicate positive relationship between spirituality and quality of life. Result also shows the difference between male and female hostlers on spirituality and quality of life.