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The present study aimed to determine the difference of stress as well as anxiety among pregnant women in different age group with special reference to their HIV test. The present study recruited a sample of sixty pregnant women (30 pregnant women below 25 years and 30 pregnant women more than 35 years of age). All the participants were administered the stress measurement inventory and the anxiety scale. To obtained data were analyzed the interpreted using statistical tool such as men, standard deviation and the t-test. The results of the present study observed that there was no significant difference in stress as well as anxiety level of pregnant women before their HIV test. Both groups of pregnant women (age group of below 25 and more than 35 years of age) do not differ statistically significant on their level of stress as well as level of anxiety.