OPEN ACCESS
PEER-REVIEWED
Original Study
| Published: December 22, 2020
Analyzation of degraded DNA samples-The need for advancement
Amity Institute of Forensic Sciences, Amity University, Noida, Uttar Pradesh, India Google Scholar More about the auther
DIP: 18.01.091/20200804
DOI: 10.25215/0804.091
ABSTRACT
DNA fingerprinting is one of the significant methods incorporated while forensic investigation. It is considered the golden standard while investigating cases involving identical disputes. The methods used earlier for analysis involved the use of RFLP or STR. The methods stated proved to be of paramount importance but had a few cons involving the higher time consumption, lacking in specificity, being a dominant marker, and most significant being the marker dominant. The issues stated added on to the problem of analysis of degraded blood samples, which is the most common evidence found on the scene of occurrence. To solve this issue, methods involving the use of cloning via gel electrophoresis by using Sequence Characterized Amplified Region technique that enhanced the performance and make it appropriate for the co-dominant gene. The techniques such as SNP’s and SCAR can be used, for analyzing the degraded blood sample, proving to be of paramount importance as a technique.
Keywords
Degraded blood sample, DNA fingerprinting, Electrophoresis, Sequence characterized amplified region technique
This is an Open Access Research distributed under the terms of the Creative Commons Attribution License (www.creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any Medium, provided the original work is properly cited.
© 2020, Chauhan I.
Received: November 20, 2020; Revision Received: November 28, 2020; Accepted: December 22, 2020
Article Overview
ISSN 2348-5396
ISSN 2349-3429
18.01.091/20200804
10.25215/0804.091
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Published in Volume 08, Issue 4, October-December, 2020